Part:BBa_K1980012:Design
pCopA MymT sfGFP with divergent CueR
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 438
Illegal NheI site found at 461 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1175
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Codon optimised for E. coli.
The linker between MymT and sfGFP is short and hydrophilic (GlySerGlySerGlySer) to allow the protein domains to fold separately.
CueR is expressed divergent form the pCopA promoter.
Source
The source organism for the MymT protein sequence is Mycobacterium tuberculosis. The CueR and pCopA components of the promoter originate from E. coli.
We ordered this part as codon optimised DNA from IDT.
References
Danya J. Martell, Chandra P. Joshi, Ahmed Gaballa, Ace George Santiago, Tai-Yen Chen, Won Jung, John D. Helmann, and Peng Chen (2015) “Metalloregulator CueR biases RNA polymerase’s kinetic sampling of dead-end or open complex to repress or activate transcription” Proc Natl Acad Sci U S A. 2015 Nov 3; 112(44): 13467–13472
Yamamoto K, Ishihama A. (2005) “Transcriptional response of Escherichia coli to external copper.” Mol Microbiol. 2005 Apr;56(1):215-27
Hötzer B., Ivanov R., Altmeier S., Kappl R., Jung G., (2011) "Determination of copper(II) ion concentration by lifetime measurements of green fluorescent protein." Journal of Fluorescence, 21(6), pp. 2143-2153. doi: 10.1007/s10895-011-0916-1